Isolation and identification of fluoride sensitive and fluoride resistant Streptococcus mutans strains from dental carries of diabetic patients using conventional methods in Sudan.

Background Dental caries also known as cavities are the most predominant disease today. Streptococcus mutans can form a biofilm on tooth surface, produce high levels of lactic acid following fermentation dietary sugars, and resistant to adverse effects low pH, properties which essential for oral micro biota. The aim this study was isolate S. characterize their antimicrobial against sodium fluoride. Fresh dental samples were collected from diabetic patients. isolates identified based acidogenic, biochemical test, properties. Methods socio-demographic data through structure designated questionnaire biological sterile cotton swabs. For analysis data, Statistical Package Social Sciences software, version 23.0 (IBM SPSS Inc., Chicago, IL) used. Results Only 32 80 (40%) gave positive growth MSBA (mitis salivarius bacitracin agar). sensitivity fluoride tested by preparing NaF four different concentrations (80, 125, 250, 500) mg\L. Fifty percent found be at concentration mg/L, while 28% 125 mg/L NaF, 16% 250 3% 500mg/L NaF. majority participants aged between 51–60 years (33%), female (54%), had type II diabetes mellitus (64%), duration less than five (35%). Conclusions By using information in study, we able discover strains that This used an key genetic mutation occurred.